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. 2015 Sep 15;26(18):3178–3189. doi: 10.1091/mbc.E15-04-0209

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© 2015 Gao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — HDAC1 regulated the transcription of miR-146a in TRAIL-treated macrophages. (A) Western blot assay for HDAC1 in mouse peritoneal macrophages stimulated with rsTRAIL for 24 h. The ratio of HDAC1 to GAPDH band intensity was normalized to the control ratio. (B) Macrophages were transfected with si-HDAC1 as indicated in Materials and Methods. After 48 h, cells were collected for Western blot analysis. The ratio of HDAC1 to GAPDH band intensity was normalized to the control ratio. (C) q-PCR assay of miR-146a expression in macrophages transfected with si-HDAC1 and treated with rsTRAIL. (D) ChIP assay for HDAC1 binding and histone H3 acetylation at the two NF-κB binding sites in the miR-146a promoter in macrophages challenged with rsTRAIL for 10 h. Data are mean ± SD (n = 3) of three independent experiments. **, p < 0.01; *, p < 0.05 compared with control.