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. 2015 Sep 15;26(18):3275–3288. doi: 10.1091/mbc.E15-02-0093

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© 2015 Koles et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Postsynaptic membrane organization is altered in past1 mutants. (A) Third-instar larval NMJ expressing myr-mRFP or mCD8-GFP under the control of BG57-GAL4. Images and insets show a single 100× spinning-disk confocal slice from muscle 4, segment A3. Arrows indicate postsynaptic foci. Scale bar, 10 μm. (B) Quantifications of myr-mRFP sum intensity and volume relative to HRP-labeled neuronal arbor volume. (C) Time-lapse of representative FRAP analysis of myr-mRFP turnover in wild-type and mutant larvae. A single spinning-disk 60× confocal slice from muscle 4, segment A3/A4, is shown. Arrows indicate progressive recovery of fluorescence from proximal to distal boutons. Scale bar, 5 μm. Quantification of FRAP recovery. (D) TEM of NMJs from wild-type and past1-mutant larvae. SSR is highlighted in yellow; red arrow indicates membrane sheets, and yellow arrow indicates membrane core. Scale bar, 1 μm. (E) past1-mutant NMJs exhibit membrane sheets. Three ∼70-nm serial sections from a past1-mutant NMJ. Scale bar, 250 nm.