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. 2015 Sep 15;26(18):3301–3312. doi: 10.1091/mbc.E15-03-0184

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© 2015 Kralt et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Localization of membrane-embedded reporter proteins encoding mammalian NLS sequences. (A) N-terminal GFP fusions of (putative) NLS regions of Sun2, Lem2, LBR, or Lap2β, the intrinsically disordered linker region (L), and first transmembrane segment (TM) of Heh2 localize to the NE-ER network, like the TM of Heh2 (GFP-TM), and do not mediate nuclear accumulation like the NLS of Heh2 (GFP-h2NLS-L-TM). (B) Whereas a C-terminally GFP fusion of a TM localizes to the NE-ER network (TM-GFP), fusion of this TM to the ID linker region of Heh2 and the NLS regions of Heh2 or Pom121 (TM-L-h2NLS-GFP and TM-L-P121NLS_290-484-GFP, respectively) results in strong NE accumulation of the protein. This accumulation is lost in the conditional Kap95 knockout. Scale bars, 5 μm.