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. 2015 Sep 15;26(18):3301–3312. doi: 10.1091/mbc.E15-03-0184

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© 2015 Kralt et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — The IBB-like region is sufficient for INM targeting, and the Pom121NLS region consists of redundant NLSs. (A) The IBB-like region of Pom121NLS alone in TM-L-P121NLS_290-326-GFP is sufficient to induce Kap95-dependent INM accumulation of the reporter protein, and (B) substitution of the lysine residues at the P2 or P2′ positions by alanine residues is sufficient to abolish NE accumulation. (C) Lysine-to-alanine mutation at the P2 and P2′ positions of the IBB-like region of the NLS of Pom121 in the context of the full Pom121NLS cluster (TM-L-P121NLS_290-484 P2/P2′-GFP) or removal of the IBB-like region from the full Pom121NLS (TM-L-P121NLS_323-484-GFP) did not alter Kap95-dependent INM accumulation of the indicated reporter proteins. Scale bars, 5 μm.