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. 2015 Sep 15;26(18):3301–3312. doi: 10.1091/mbc.E15-03-0184

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© 2015 Kralt et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Pom121NLS rescues localization of Heh2ΔNLS and synthetic sickness of Heh2ΔNLS,nup84Δ double mutant. (A) Deconvolved wide-field images of yeast expressing native levels of GFP-Heh2 with wild-type NLS (h2NLS), without the NLS (Δh2NLS), or with Pom121NLS_290-326 instead of the h2NLS (Δh2NLS, Pom121NLS_290-326). Scale bar, 5 μm. (B) Synthetic sick/lethal interaction using tetrad dissection of nup84Δ expressing wild-type (h2NLS) and mutant variants (Δh2NLS and Δh2NLS, Pom121NLS_290-326) or no Heh2 (Heh2Δ). Each tetrad is oriented vertically and represents the meiotic progeny of a heterozygous diploid between GFP-HEH2-NAT/NUP84 and HEH2/nup84::KANMX. Two representative tetrads for each double mutant are shown. The genetic background of each spore is identified by the presence of the NAT or KAN marker, respectively. The double-mutant spore colonies are enclosed in circles, single mutants are enclosed in squares or diamonds, and wild-type strains are not enclosed.