FIGURE 6:

In the absence of Cbl, Mop function is dispensable. (A–A′′) Knockdown of cbl in flipout clones (marked with GFP) causes mild accumulation of Wg and Hrs in the wing disk. (B–B′′) cbl; mop double knockdown in clones causes a sharp increase in Hrs puncta (B′′) without affecting Wg trafficking (B′). Colocalization of Hrs and Rab5 (C–C′′), Hrs and Rab7 (D–D′′), and Hrs and Rab11 (E–E′′) in the wing disk after knockdown of cbl in flipout clones (marked with GFP). (F) Quantification of colocalization of Hrs and markers was done by measuring Pearson’s coefficient in control and cbl; mop double-knockdown tissue. Bar graph shows continued localization of Hrs in Rab7-positive late endosomes. p < 0.001 for Hrs and Rab5, p < 0.01 for Hrs and Rab7, and p < 0.002 for Hrs and Rab11. mop was knocked down using VDRC line 104860. Scale bars, 20 μm.