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. 2015 Sep 15;26(18):3359–3371. doi: 10.1091/mbc.E15-01-0037

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© 2015 Nagiec, McCarter, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Dynamics of differentially phosphorylated forms of Fus3. (A) Left, wild-type cells were treated for the indicated times with 10 μM α-factor and resolved by Phos-tag SDS–PAGE and immunoblotted with Fus3 antibodies (top) or G6PDH load control antibodies (bottom). Right, dual-phosphorylated (ppFus3), monophosphorylated (pFus3), and nonphosphorylated (npFus3) quantified as a percentage of total Fus3 at 15 min. Results are reported as ±SEM (n ≥ 3). (B and C) Wild-type (WT), ste5^FB (feedback-phosphorylation deficient), ste5^ND (nondocking), ptp2Δ/ptp3Δ, and msg5Δ (phosphatase-deficient) mutants treated and resolved by Phos-tag SDS–PAGE, as described. Dual-phosphorylated Fus3 is quantified as a percentage of total Fus3 at 15 min. Results are reported as ±SEM (n ≥ 3).