TABLE 4:
Model rate parameters.
| Parameter | Complete model | Limiting case 1 | Limiting case 2 | Description |
|---|---|---|---|---|
| k1 | 1.15 × 101 | 8.51 | 7.71 | [Ste5m] activation |
| k2 | 1.25 × 10−4 | 1.12 × 10−4 | 2.43 × 10−2 | [Ste5m] deactivation |
| k2p | 1.66 × 10−2 | 1.1 × 10−4 | 2.43 × 10−2 | [pSte5m] deactivation |
| k3 | (2.73 × 10−4, 4.09 × 10−5) | (1.18 × 10−4, 1.77 × 10−5) | (1.5 × 10−4, 2.25 × 10−5) | [Ste5m] feedback phosphorylation |
| k3p | (4.81 × 10−2, 7.21 × 10−3) | (1.43 × 10−2, 2.15 × 10−3) | (4.78 × 10−2, 7.17 × 10−3) | [Ste5m] feedback phosphorylation |
| kb | 1.29 × 10−4 | 8.2 × 10−2 | 2.55 × 10−3 | [Fus3] dephosphorylation |
| k4 | 4.36 × 10−1 | 4.38 × 10−1 | 4.88 × 10−1 | [p-Ste5m] dephosphorylation |
| k5 | 1.15 | 1.48 | 1.32 | [Fus3] synthesis |
| k5p | 0.0 | 0.0 | 0.0 | |
| k6 | 2.64 × 10−3 | 2.49 × 10−3 | 3.26 × 10−3 | [Fus3] degradation |
| k7 | 1.43 × 10−1 | 1.46 × 10−1 | 1.94 × 10−1 | [pp-Fus3] phosphorylation |
| k7p | 9.13 × 10−2 | 1.46 × 10−1 | 7.73 × 10−1 | [pp-Fus3] feedback phosphorylation |
| k8 | 6.01 × 10−4 | 6.71 × 10−4 | 0.0 | [pp-Fus3] dephosphorylation |
| k9 | 1.16 × 10−3 | 1.64 × 10−3 | 0.0 | [p-Fus3] dephosphorylation |
| k10 | 5.19 × 10−4 | 1.06 × 10−3 | 1.14 × 10−3 | [Fus3] synthesis |
| k11 | 5.16 × 10−3 | 4.06 × 10−4 | 3.28 × 10−3 | [Msg5] synthesis |
| k12 | 1.09 × 10−4 | 5.39 × 10−4 | 1.11 × 10−4 | [Msg5] degradation |
| kms | (2.04 × 10−3, 0.) | (3.19 × 10−3, 0.) | (2.82 × 10−4, 0.) | [Msg5] dephosphorylation |
| kamsg5 | 1.06 × 10−4 | 2.16 × 10−2 | 1.09 × 10−4 | [Msg5] activation |
| K1 | 2.5 × 10−1 | 2.45 × 101 | 2.47 × 101 | Michaelis constant |
| K2 | 3.1 | 1.31 × 101 | 2.84 | Michaelis constant |
| K3 | 4.12 | 1.04 | 1.03 | Michaelis constant |
| K4 | 1.07 | 2.49 × 101 | 1.16 | Michaelis constant |
| Kmsg5 | 1.62 | 1.36 | 2.71 | Michaelis constant |
The name, numerical value, and function of each rate parameter in the model are given. The rate parameters k3 and k3p are shown for the wild-type (left), ste5ND (right), and msg5Δ plus ste5ND (right) models. The rate parameter kms is shown for the wild-type (left) and msg5Δ plus ste5ND (right) models. Parameters k3 and k3p are “knocked down” in the model and are assumed to take 5% of the original value for both the ste5ND and ste5ND plus msg5Δ mutant models. Parameter kms is set to 0 for both the msg5Δ and ste5ND plus msg5Δ mutant models. Parameters k5p and K2 have no appreciable affect on the model and are set to 0 in the model figures.