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. 2015 Sep 14;10(9):e0137465. doi: 10.1371/journal.pone.0137465

Fig 4. RhoGAP18B isoforms inhibit different members of the Rho-family of GTPases.

Fig 4

(A-C) Western blots of GTPase activation experiments. Activated Cdc42 and Rac1 GTPases were pulled down with Pak-PBD, while activated Rho1 was pulled-down by Rhotekin-RBD from S2 cell lysates and then blotted with anti-GTPase antibodies. Incubation of cells with isoform-specific RhoGAP18B RNAi is indicated atop. Representative blots of pull-down assays are shown, and each assay was repeated 5–7 times. (D) Quantitation of active/total GTPase, normalized to untreated S2 cells, suggests specific (PA-Cdc42, and PD-Rac1), as well as general (PC) GTPase activating activities. (One sample t-test with Bonferroni post-hoc comparison vs. control S2 cells; n = 5–7, * p < 0.05; t > 3.4).