Table 2. qPCR validation of DEGs obtained from RNA-seq.
Gene | Contrast | qPCR 1 | RNA-seq 2 |
---|---|---|---|
RPL29 | WRRh Vs. WRRl | 1.58 | 1.05 |
XHh Vs. XHl | 1.09 | 1.04 | |
WRRh Vs. XHh | 1.42 | 0.90 | |
WRRl Vs. XHl | 0.98 | 0.89 | |
PDK4 | WRRh Vs. WRRl | 7.26 | 4.60 |
XHh Vs. XHl | 3.03 | 1.94 | |
WRRh Vs. XHh | 4.89 | 13.96 | |
WRRl Vs. XHl | 2.04 | 5.90 | |
FOXO3 | WRRh Vs. WRRl | 7.82 | 2.18 |
XHh Vs. XHl | 0.76 | 0.98 | |
WRRh Vs. XHh | 11.13 | 6.56 | |
WRRl Vs. XHl | 1.08 | 2.96 | |
LAPTM5 | WRRh Vs. WRRl | 11.47 | 2.18 |
XHh Vs. XHl | 2.18 | 1.39 | |
WRRh Vs. XHh | 4.63 | 0.32 | |
WRRl Vs. XHl | 0.88 | 0.20 |
1meant the ratio of expression value (2-ΔΔCt) in the second group to the first group,
2fold change between contrasts presented (the ratio of FPKM in the second group to the first group).