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. 2015 Sep 14;11(9):e1005472. doi: 10.1371/journal.pgen.1005472

Fig 2. Mutants lacking msDNA are defective for anaerobic growth.

Fig 2

(A-B) Overnight cultures of WT (closed circles; HA420), ΔSTM3846 (A; JE122) or Δmsd (B; JE144) with empty vector (open squares), and complemented ΔSTM3846 (A; HA1446) or complemented Δmsd (B; JE145) mutant (open diamonds) were transferred into an anaerobic chamber, sub-cultured 1:100 into LB broth pre-incubated for 24 hours in anaerobic conditions, and incubated at 37°C. CFU were determined hourly. Data shown are mean +/- SEM of 3 independent experiments. (C-D) Aerobic growth of WT (closed circles; HA420) and ΔSTM3846 mutant (open squares; HA1444) in (C) LB and (D) M9 minimal media. (E-F) Aerobic growth of WT (closed circles; HA420) and Δmsd mutant (open squares; JE135) in (E) LB and (F) M9 minimal media. Each data point is the mean +/- SEM of three independent experiments.