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. 2015 Sep 14;11(9):e1005472. doi: 10.1371/journal.pgen.1005472

Fig 6. Growth defects of the ΔSTM3846 mutant in the presence of electron acceptors are linked to disruption of alternate electron acceptor reductases.

Fig 6

Bacterial cultures were grown anaerobically for 4 hours in the presence of alternate electron acceptors as indicated in Fig 5. (A) Anaerobic growth of the WT (HA420), a ΔSTM3846 mutant (HA1444), a mutant lacking all three nitrate reductases (ΔnarG ΔnarZ ΔnapA; CAL50), and a ΔnarG ΔnarZ ΔnapA ΔSTM3846 quadruple mutant (JE335) in the absence (black bars) or presence (white bars) of nitrate. (B) Anaerobic growth of the WT (HA420), a ΔSTM3846 mutant (JE142), a mutant lacking the fumarate reductase (ΔfrdA; JE309), and a ΔfrdA ΔSTM3846 double mutant (JE334) in the absence (black bars) or presence (white bars) of fumarate. (C). Anaerobic growth of the WT (HA420), a ΔSTM3846 mutant (HA1444), a mutant lacking all three putative DMSO reductases (ΔSTM0964 ΔSTM2530 ΔSTM4305; JE341), and a ΔSTM0964 ΔSTM2530 ΔSTM4305 ΔSTM3846 quadruple mutant (JE343) in the absence (black bars) or presence (white bars) of DMSO. Bars represent mean +/- SEM of at least three independent experiments. * Indicates significant difference between mutant and WT for that condition (P<0.05 by ANOVA). ** Indicates significant difference between conditions (P<0.05 by t-test).