Table 4. Forward and reverse sequence for all primer pairs used to validate putative SNPs in genotypes Albion and Voyager.
P. vulgaris genome ID | Gene | Forward primer | Reverse primer | Product Size (bp) |
---|---|---|---|---|
Phvul.008G157800 | PvYSL | CGCTATGTCGTAACACTTCTGCACC | TTTGTGCTTGCTGCCTTAGGTGGG | 741 |
PvYSL | GGGAAGGGCAGAAAAGCCTTCGAC | TTGCCTTGATCCTCGGTGATGGGT | 874 | |
Phvul002G19000 | PvHMA | AACCTCTCACCGCGACCTCACTAC | CCCACACAACAACCCCATCGGAAG | 1483 |
PvHMA | AACCTCTCACCGCGACCTCACTAC | CATGTTCGCAGATCCACGGCGTAA | 1131 | |
PvHMA | GACACGGCGGTTTTGCTGACTTTG | GCACTCTCTAATGCCTGCCCTCCT | 746 | |
PvHMA | GTTGGTGCATCTCAGGGTGTGCTC | GGCCAATGGATGCTCACTATTCACCT | 853 | |
Phvul.011G173100 | PvZIF | GCCCAGCATTGGGAGGCTATTTGG | AAGCCACATTCGGAACATGACCGC | 1211 |
PvZIF | CGTGACGTGTGCAATGATGCCACT | CGCACCAACAACACAAAACAGGGA | 561 | |
PvZIF | AGGTGGTGCAGTGTGAGTGTTCCT | AGGCAAGTTACAGATTGAATTGGTTCCCT | 886 |