Table 4. Relative proportions of different forms of plasmid DNA after 1-Fe, 2-Fe and 5-Fe treatments in the presence of groove binders and ROS scavengers.
| Proportion [%] | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| 1-Fe | 2-Fe | 5-Fe | |||||||
| Form*: | I | II | III | I | II | III | I | II | III |
| Untreated | 89.2 | 10.8 | 0 | 99.8 | 0.2 | 0 | 98.1 | 1.9 | 0 |
| H 2 O 2 | 59.3 | 40.7 | 0 | 95.5 | 4.5 | 0 | 94.7 | 5.3 | 0 |
| Complex + H 2 O 2 | 0 | 82.4 | 17.6 | 0 | 84.0 | 16.0 | 0 | 56.9 | 43.1 |
| Complex + H 2 O 2 + Hoescht | 0 | 86.8 | 13.2 | 0 | 63.7 | 36.3 | 0 | 52.8 | 47.2 |
| Complex + H 2 O 2 + Methyl green | 0 | 90.7 | 9.3 | 0 | 78.6 | 21.4 | 0 | 51.0 | 49.0 |
| Complex + H 2 O 2 + Tiron | 47.6 | 52.4 | 0 | 60.7 | 39.3 | 0 | 0 | 96.7 | 3.3 |
| Complex + H 2 O 2 + Sodium Azide | 41.8 | 58.2 | 0 | 63.6 | 36.4 | 0 | 54.5 | 45.5 | 0 |
| Complex + H 2 O 2 + DMSO | 57.5 | 42.5 | 0 | 26.0 | 62,7 | 11.3 | 0 | 74.8 | 25.2 |
* I: supercoiled form, II: open form, III: linear form.