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. 2015 Sep 14;10(9):e0137924. doi: 10.1371/journal.pone.0137924

Fig 3. LCN2 enhances mRNA expression of M1 macrophage markers.

Fig 3

(A) mRNA expression of the M1 macrophage markers TNF-α, iNOS, IL-6 and CCL5 as well as the M2 macrophage markers Ym1/2 and Arg1 in murine primary BMDM following LCN2 (0.5 μg/mL) stimulation was determined by real time PCR at the indicated time points. HPRT was used as housekeeping gene and the relative mRNA expression was calculated using the 2-ΔΔCT method. con = control. *P<0.05, **P<0.01 vs. control, n = 5–10 replicated experiments. (B) Release of TNF-α from murine primary BMDM following LCN2 (0.5 μg/mL) stimulation for 6 hours was assessed by ELISA. *P<0.05 vs. control, n = 12. (C) Protein expression of the M2 macrophage markers Ym1 and Arg1 following LCN2 (0.5 μg/mL) stimulation for 6 hours was determined by Western blot. GAPDH was used as loading control. n = 3–4 replicated experiments.