Table 1. Working concentrations and mechanisms of action for each of the pharmacological agents that yielded the highest percent change in OCR compared to basal OCR for D. rerio and F. heteroclitus.

Compound	Mechanism of Action	Working concentration (mM) for Danio rerio	Working concentration (mM) for Fundulus heteroclitus
FCCP	Protonophore and un-coupler of oxidative phosphorylation, depolarizes the mitochondrial membrane	0.002 in1% DMSO	0.005
Sodium azide	Inhibits cytochrome c oxidase (Complex IV)	100 a	30 a
Antimycin A + rotenone	Antimycin A: inhibits cytochrome c reductase (Complex III), Rotenone: inhibits NADH dehydrogenase (Complex I) (This mixture can be used instead of sodium azide)	0.044 + 0.044	0.044 + 0.044
Oligomycin	Inhibits the proton channel of ATP synthase (Complex V), blocking oxidative phosphorylation of ADP to ATP	0.010 in 1% DMSO	0.015–0.025 in 1% DMSO a

^aAt these concentrations oligomycin and NaN₃ yielded a ~30% decrease in OCR compared to basal OCR.