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. 2015 Sep 14;10(9):e0138131. doi: 10.1371/journal.pone.0138131

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© 2015 Macedo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Representative images of confocal microscopy of ovine macrophages expressing LAMP1 and infected with WT-mCherry, ΔabcBA-mCherry and ΔvirB2-mCherry strains. (B) Average percentage of BCVs that were positive for LAMP1 during infection. (C) Average number of bacteria per cell (total of 100 cells per time point) during the course of the infection. (D) Kinetic infection of WT-mCherry, ΔabcBA B. ovis-mCherry, and ΔvirB2 B. ovis-mCherry in ovine macrophages expressing LAMP1 by transient transduction (see Material and Methods section). Data represent mean and standard error of at least 100 cells from three independent experiments (** p<0.01; *** p<0.001).