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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Transl Res. 2015 Mar 16;166(4):384–398. doi: 10.1016/j.trsl.2015.03.002

Figure 2.

Figure 2

2A. Heterodimer CLCF1-CRLF1 complex did not increase Palb. Isolated rat glomeruli were incubated with recombinant CLCF1-CRLF1 (0.1–10ng/mL) for 15 minutes at 37°C. CLCF1-CRLF1 did not affect Palb at any of the concentrations used. N=15 glomeruli from 3 rats (5 glomeruli from each rat) in each group.

2B. Heterodimer CLCF1-CRLF1 heterodimer blocked the effect of CLCF1 on Palb. Isolated rat glomeruli were pre-incubated with CLCF1-CRLF1 (5–20ng/mL) for 15 minutes followed by addition of CLCF1 (5ng/mL) for 15 minutes at 37°C. CLCF1 alone caused a significant increase in Palb (#, P<0.001 vs. control or CLCF1-CRLF1 alone) and pre-treatment with CLCF1-CRLF1 blocked the effect of CLCF1 in a dose-dependent manner (*, P<0.02 vs. CLCF1 alone; **, P<0.005 vs. CLCF1 alone). N=15 glomeruli from 3 rats (5 glomeruli from each rat) in each group.