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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Transl Res. 2015 Mar 16;166(4):384–398. doi: 10.1016/j.trsl.2015.03.002

Figure 3.

Figure 3

3A. Pre-treatment of glomeruli with CLCF1-CRLF1 heterodimer blocked the effect of FSGS serum on Palb. Isolated glomeruli were pre-incubated with CLCF1-CRLF1 complex (5–50ng/mL) for 15 minutes followed by addition of FSGS serum (20µL/mL) and incubation for 15 minutes at 37°C. FSGS serum alone caused a significant increase in Palb (#, P<0.001 vs. Control) that was blocked by 10ng/mL (*, P<0.02 vs. FSGS alone), 20ng or 50ng/mL (**, P<0.005 vs. FSGS alone). N= 15 glomeruli from 3 rats (5 glomeruli from each rat) in each group.

3B. Pre-treatment with FSGS serum prevented the protective effect of CLCF1-CRLF1 heterodimer on Palb. Isolated glomeruli were pre-incubated with FSGS serum (20µ/mL) for 15 minutes at 37°C followed by addition of CLCF1-CRLF1 (5–50 ng/mL) for 15 minutes at 37°C. FSGS serum with or without CLCF1-CRLF1 increased Palb significantly (*, P<0.001 vs. control). N= 15 glomeruli from 3 rats (5 glomeruli from each rat) in each group.