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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Brain Struct Funct. 2015 Mar 14;221(4):2375–2383. doi: 10.1007/s00429-015-1020-0

Fig. 4.

Fig. 4

Dual IFL for OT (Alexa-647, imaged in green channel) and GLP1 (TSA-Cy3, imaged in red channel) in a 2.0-mm-thick coronal slab through the diencephalon in a juvenile male rat (P24) perfused with Hydrogel solution B. This image is a maximum intensity Z-projection obtained from 268 2.0 µm optical sections (i.e., 536 µm thick). The total image volume is 0.127 mm3 (i.e., 410 µm × 579 µm × 536 µm). 3V third ventricle, D dorsal, M medial. Scale bar 100 µm (color figure online)