Table 1.
Reagents and commercial sources
Reagent | Source, product # |
---|---|
40 % Acrylamide solution | Bio-Rad, 161-0140 |
10× Phosphate-buffered saline, pH 7.4 | Invitrogen, GIBCO 10010 |
2,2′-Azobis[2-(2-imidazolin-2-yl) propane] dihydrochloride (VA-044 initiator) | Wako, 27776-21-2 |
Paraformaldehyde aqueous solution, 16 % | Electron Microscopy Sciences, 15710-S |
Boric acid | Sigma–Aldrich, B3637 |
Sodium dodecyl sulphate (SDS) | Promega, H5114 |
2,20-Thiodiethanol | Sigma–Aldrich, 166782 |
Acrolein | Polysciences, 00016 |