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. 2015 Sep 14;10(9):e0137947. doi: 10.1371/journal.pone.0137947

Fig 3. Bcr1 antagonizes Hsp90’s effect on filamentation.

Fig 3

(A) Growing strains with wild type and reduced levels of Hsp90 under conditions that favour yeast growth (YPDM, 30°C) results in filamentation upon Hsp90 compromise. Deletion of BCR1 enhances filamentation induced by Hsp90 depletion. (B) Deletion of BCR1 alone does not affect filamentation under canonical filament-inducing conditions. Cells were imaged after 2 hours (YPD + 10% serum) and 6 hours (Lee’s and Spider media). (C) qRT-PCR measuring expression levels of PCL1, PHO85, and HMS1 in wild type and bcr1∆/∆ mutant strains grown in non-filament inducing conditions at 30°C. Bcr1 represses expression of genes in the PHO85-PCL1-HMS1 pathway (* = P < 0.05, ** = P < 0.01, *** = P < 0.001).