Fig 5. BHQ (Bromo, HAT and poly Glutamine stretch) domain is required for neuroprotective function of CBP.
In third instar eye imaginal disc, TUNEL staining was employed to mark the dying cells. In comparison to GMR>Aβ42 background, (A, A’) the number of dying cells marked by TUNEL are significantly reduced when CBP full length is misexpressed along with Aβ42 (GMR> Aβ42 +CBP FL) in the Drosophila eye. (B-E) The number of dying cells marked by TUNEL staining in (B, B’) GMR>Aβ42 +CBP∆Q, (C, C’) GMR> Aβ42 +CBP∆HQ, (D, D’) GMR>CBP∆NZK, (E, E’) GMR> Aβ42 +CBP∆Kix background were comparable to (A, A’) GMR> Aβ42 +CBP FL background. However, the number of dying cells marked by TUNEL staining in (F, F’) GMR> Aβ42 +CBP∆BHQ and (G, G’) GMR> Aβ42 +CBPRNAi were significantly increased. (H) Graph comparing the number of dying cells in the all the backgrounds tested. The number of TUNEL cells counted from five imaginal disc of all backgrounds were used for 2-tailed test and P values (< .05). These rescue phenotype based on number of TUNEL positive cells counted are significant. Magnification of all eye imaginal discs is 20X.