Table 1. Sequence and assembly statistics of six cotton libraries with/without A. flavus infection.
| Tissue/library | Pericarp | Seed | |||||
|---|---|---|---|---|---|---|---|
| Parameter | NIP* | NTP | TP | NIS | NTS | TS | Total |
| # Raw single-end reads | 163,090,907 | 138,340,968 | 196,958,025 | 193,242,949 | 137,348,154 | 82,059,811 | 911,040,814 |
| Bases sequenced (Gbp) | 16.30 | 13.83 | 19.69 | 19.32 | 13.73 | 8.205 | 91.1 |
| Sequence coverage | 65.24X | 55.34X | 78.78X | 77.3X | 54.94X | 32.82X | 364.42X |
| # Cleaned reads used in assembly | 162,591,684 | 137,657,801 | 196,080,070 | 192,530,847 | 136,780,468 | 81,716,978 | 907,357,848 |
| # Assembled unique transcripts | 99,772 | 107,294 | 122,657 | 100,510 | 82,896 | 73,440 | 586,569 |
| # unigenes | 31,425 | 35,831 | 40,010 | 31,635 | 28,019 | 23,638 | 190,558 |
| Transcript N50 (bp) | 1887 | 1842 | 1976 | 1927 | 1703 | 1787 | 1864 |
| Transcript N90 (bp) | 701 | 647 | 699 | 705 | 634 | 658 | 675 |
| Maximum transcript size (bp) | 15441 | 14102 | 15414 | 15383 | 10154 | 14933 | 15441 |
| Minimum transcript size (bp) | 201 | 201 | 201 | 201 | 201 | 201 | 201 |
| Average transcript Size (bp) | 1339.58 | 1275.85 | 1351.4 | 1353.22 | 1217.53 | 1278.19 | 1307.8 |
| Transcriptome size (Mbp) | 133.65 | 136.89 | 165.75 | 136.01 | 100.92 | 93.87 | 767.11 |
*NIP = non-inoculated pericarp
NTP = atoxigenic pericarp; TP = toxigenic pericarp; NIS = non-inoculated seed; NTS = atoxigenic seed; TS = toxigenic seed.