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. 2015 Sep 14;11(9):e1005162. doi: 10.1371/journal.ppat.1005162

Fig 5. Functional consequences of absence of glycosylation in class II strains.

Fig 5

(A) Impact of class II pilin glycosylation on adhesion to endothelial cells. Strains 8013, LIM707, LIM534, FAM20 and their corresponding pglD mutants were allowed to adhere to endothelial cells for 4 hours and the number of adherent bacteria analyzed. A non-piliated pilD mutant of the 8013 strain was used as a negative control. (B-D) Quantitative and qualitative impact of pilin glycosylation on bacterial aggregation in three class II pilin-harboring strains: (B) FAM20, (C) LIM707 and (D) LIM534. Bacteria were allowed to aggregate in suspension and the size and number of aggregates determined using fluorescence microscopy following DAPI staining. The scale bar indicates 200 μm. Mean ± standard deviation was determined and indicated on the figures. Statistical analysis was done by paired two-tailed t-test; * indicates P ≤ 0.05; ** indicates P ≤ 0.01.