Table 5. Oligonucleotides used in this study.
| Primer | Sequence* | Enz. |
|---|---|---|
| pglHNF2 | GTCGACGGAATTTCATTTCCGGAAAAC | SalI |
| pglHNR | GGATCCAGCAATAAGGGGCGACGATG | BamHI |
| pglHCF | GGATCCAAGTTGCCGAAGTCCTTAC | BamHI |
| pglHCR | GAGCTCACCGCCAGATTGAAAATGC | SacI |
| E707F | GAGGCATTTCCTTTCCAATTAGGAGTAATTTTATGAAAGCAATCCAAAAAGGTTTC | |
| E707R | CCATTTATTATTTCCTTCCTCTTTTCTGATCCTTACTTATTTGGTGCGGCAGGTAGA | |
| 8013-lpxC-pilE_FWD | GCCGTCTGAAGCGTCGGGCAAATCATCGC | |
| 8013-lpxC-pilE_REV | GATTGTGATTGCCATCGTCG | |
| FAM20-pilE_FWD | gccgtctgaaATTTGGTGCGGCAGGTAG | |
| FAM20-pilE_REV | AGGTTTCACCCTGATCGAG | |
| McPilRBS | gcatttcctttccaattaggag | |
| 3-end-ErmR_FWD | cgttatgaaatgggttaaca | |
| Mid_pilEandS1FWD | GTCAGCAGTGCCGAAAATTGTCAG | |
| Late_pilEandS1FWD | CCTCGTCGGTGCAGAAACTTA | |
| 3’end_pilS1 FWD | ctcggtgacggctgatttttgac | |
| 5’END_FKBP REV | gctgaaagtgtacgaataaAGC | |
| 3’END_PILS5 REV | CTGACATAAtggcttcaag | |
| 3END8013REVPILS1 | CATCCTTTTGGTCgaaggtc | |
| 3END8013FORPILS1 | gaccttcGACCAAAAGGATG | |
| 3END8013REVPILS2 | GACGAAGCTATCCTTttggccg | |
| 428BP_PRE-PILS1 FOR | CATCGGTACGGAAACTTATCG | |
| FAM18-PILE_FWD | gccgtctgaaATTTGGTGCGGCAGGTAG | |
| FAM18-PILE_REV | AGGTTTCACCCTGATCGAG |
* Restriction sites in bold