Table 2.

Numerical data as obtained from PCR amplification by ISSR primers among Ocimum species

Sr. no.	Locus name	Sequence 5′–3′	GC content (%)	Tm	Molecular weight (bp)	No. of loci	No. of polymorphic loci	Percent polymorphism (%)	PIC value
1	UBC 443	ACACACACACACACACACT	47	49	453–2,159	18	17	94.44	0.909
2	UBC 807	AGAGAGAGAGAGAGAGT	47	45	89–1,556	22	22	100	0.930
3	UBC 808	AGAGAGAGAGAGAGAG(CT)C	53	51	604–2,224	13	12	92.30	0.892
4	UBC 818	CACACACACACACAG	53	42	342–1,835	18	17	94.44	0.915
5	UBC 825	ACACACACACACACACT	47	45	446–2,279	20	20	100	0.934
6	UBC 834	AGAGAGAGAGAGAGAG(CT)T	47	49	392–2,597	19	19	100	0.934
7	UBC 840	GAGAGAGAGAGAGAGAYT	47	45	222–1,952	18	18	100	0.919
8	UBC 841	GAGAGAGAGAGAGAGAYC	53	47	189–2,940	22	22	100	0.937
9	UBC 855	ACACACACACACACACYT	47	45	238–2,284	15	15	100	0.908
10	ISS RA7	AGAGAGAGAGAGAGAGAGAGT	48	52	254–1,720	21	21	100	0.930
11	ISS RA12	GAGAGAGAGAGACC	57	40	225–1,984	32	31	96.88	0.943
12	CTC 4RC	CTCCTCCTCCTCRC	69	44	307–2,587	20	20	100	0.922
Total					–	238	234	–	–
Average					313–176	19.8	19.5	98.17	0.923