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Phylogenetic tree of annotated sequences of 16S rRNA gene cloned directly from environmental DNA. The analysis involved 71 nucleotide sequences aligned with Clustal W (Thompson et al. 1994) and the evolutionary history was inferred by maximum parsimony method from MEGA6 programs (Tamura et al. 2013) using 1,000 bootstrap replicates. Unknown sequences are represented by a clone number
