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. 2015 Jul 16;24(5):921–927. doi: 10.1007/s11248-015-9897-1

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Blastocyst PCR reaction for Lrrc71^{tm1a(KOMP)Wtsi} using three primers in multiplex to amplify the WT (515 bp) and mutant (258 bp) alleles in the same reaction. Samples are visualised on a QIAxcel Advanced System (Qiagen). Genotype calls are shown above each sample lane and the last three lanes contain negative controls