| dNTPS | (2 mM) | 3 μl |
| PCR BUFFER 10× | 3 μl | |
| PRIMER 1 | (0.5 μM/μl) | |
| PRIMER 2 | (0.5 μM/μl) | |
| PRIMER 3 | (0.5 μM/μl) | |
| H2O | ||
| TAQ POLYMERASE | (5 U/μl) | 0.3 μl |
| DNA | (5 μl of lysate) | 5 μl |
| TOTAL | 30 μl |
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