Figure 2. BeCyclOp photoactivation and cGMP production.
(a) BeCyclOp action spectrum. Dotted grey line indicates the relative dark activity, light intensities at different wavelengths were adjusted to ∼0.02 mW mm−2. cGMP production per photon amount was calculated, normalized to the action spectrum peak. Mean of N=3 experiments, error bars, s.d. (b) Light (532 nm) intensity dependence of mean normalized cGMP production of BeCyclOp-containing membranes; K0.5=0.055 mW mm−2. N=3 experiments, error bars, s.d. (c) Kinetics of mean, normalized cGMP production of BeCyclOp-containing membranes, measured at indicated times after 20 ms illumination. Fitting a mono-exponential yields τ=320±20 ms. n=5 experiments; error bars, s.d. (d) Mean BeCyclOp cGMP production in the dark and with continuous illumination (0.5 mW mm−2, 532 nm), measured at indicated times. N=3 experiments, error bars, s.d.