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qRT-PCR analysis was performed in samples from islets collected at the indicated postnatal days in rats weaned at P15 (EW, dotted line) or at P21 (NW, solid line) with chow diet. miRNA levels were normalized to U6 and expressed as fold change. Results correspond to means±s.d. (n=4–8 per group). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 are determined by ANOVA comparing EW with NW at the indicated stage.
