Table 1. Cloning efficiency of different target sequences and vectors.
| Target | Vector | Number of positive colonies | Number of total colonies | Average positive rate±s.d. (%) |
|---|---|---|---|---|
| E. coli-lacZ 50 kb | pCC1BAC | 28±4 | 42±8 | 65.8±4.0 |
| E. coli-lacZ 50 kb (R) | pCC1BAC | 26±8 | 41±7 | 62.2±9.6 |
| E. coli-lacZ 75 kb | pCC1BAC | 8±3 | 23±8 | 35.1±1.6 |
| E. coli-lacZ 100 kb | pCC1BAC | 5±3 | 28±15 | 21.5±11.2 |
| E. coli-lacZ 150 kb | pCC1BAC | 1* | 51* | 1.9* |
| E. coli-sfGFP 50 kb | pCC1BAC | 25±6 | 47±12 | 55.2±12.4 |
| E. coli-sfGFP 75 kb | pCC1BAC | 12±4 | 29±5 | 39.2±6.4 |
| E. coli-sfGFP 100 kb | pCC1BAC | 7±3 | 35±12 | 21.6±7.4 |
| B. subtilis-pks 78 kb | pCC1BAC | 4±1 | 34±9 | 12.2±3.7 |
| S. venezuelae-jad 36 kb | p15A | 60±4 | 66±10 | 90.5±6.1 |
| S. aureofaciens-ctc 32 kb | p15A | 70±3 | 78±6 | 90.8±3.7 |
All experiments were performed in triplicates.
*Only one positive colony was obtained in three independent trials.