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. 2015 Sep 3;6:8143. doi: 10.1038/ncomms9143

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(a) Protein expression levels for double mutant single chain (SC-DM) and triple mutant single chain (SC-TM) were compared with DS-Cav1 (ref. 10) in cell culture supernatants 72 h post transfection and (b) the fraction of RSV F protein binding to prefusion-specific CR9501 antibody after storage at 4 °C for indicated days. Bars or symbols represent average from two measurements, lines represent range of values. On (b) prefusion F fraction at day of harvest (day 0) is normalized to 1.