Table 3. Summary of kinetochore protein copy number following the protein depletions in Figs 3b (a), 4b (b) and 6b (c) based on the control values in Table 2, and the percentage of control levels from Figs 3b, 4b and 6b.
| (a) | Hec1 | CENP-C | CENP-T |
| Control | 244 (100±17%) | 215 (100±29%) | 72 (100±15%) |
| CENP-T RNAi | 90 (37±7%) | 213 (99± 28%) | 4 (5±2%) |
| CENP-C RNAi | 100 (41±7%) | 6 (3±4%) | 44 (61±13%) |
| (b) | CENP-T | CENP-C | Dsn1 | Knl1 |
|---|---|---|---|---|
| Control | 72 (100±23%) | 215 (100±19%) | 151 (100±26%) | NA (100±16%) |
| CENP-T RNAi | 4 (5±3%) | 204 (95±23%) | 79 (52±11%) | NA (50±10%) |
| CENP-C RNAi | 42 (58±12%) | 15 (7±4%) | 44 (29±7%) | NA (29±6%) |
| (c) | Hec1 | CENP-C | CENP-T | EGFP |
KMN network proteins |
|
| Dsn1 | Knl1 | |||||
| Control | 244 (100±16%) | 215 (100±29%) | 72 (100±15%) | 0 (0±0%) | 151 (100±16%) | NA (100±14%) |
| CENP-C RNAi | 100 (41±7%) | 6 (3±4%) | 38 (53±12%) | 0 (0±0%) | 30 (20±5%) | NA (20±5%) |
| Chimera1 (CENP-C RNAi) | 268 (110±23%) | 0 (0±10%) | 38* (53±12%) | 92 (100±17%) | 100 (66±12%) | NA (58±10%) |
NA, not applicable; RNAi, RNA interference. All values=mean±s.d.
(a) Mean values of kinetochore intensities normalized by control values for CENP-T, CENP-C and Hec1 (Fig. 3b). (b) Mean values of kinetochore intensities normalized by control values for CENP-T, CENP-C, Dsn1 and Knl1 (Fig. 4b). (c) Mean values for immunofluorescence intensities at kinetochores for CENP-C, Hec1, Dsn1 and Knl1 normalized by control values in CENP-C RNAi-treated cells and EGFP–chimera1 cells (Fig. 6b).
*This value is assumed to be equal to CENP-C RNAi value (see text).