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. 2015 Sep 15;9:353. doi: 10.3389/fncel.2015.00353

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Copyright © 2015 Damulewicz, Loboda, Bukowska-Strakova, Jozkowicz, Dulak and Pyza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression in the retina of different strains of D. melanogaster. A—per gene; B—tim; C—clk, D—cry, E—Atpα, F—nrv2, G—brp. Tissues were collected at five time points: ZT1, ZT4, ZT13, ZT16, ZT20 in LD12:12 and CT1, CT4, CT13, CT16, CT20 in DD. Relative quantitation (RQ) determines the changes in steady-state mRNA level, data are normalized to ZT1 in LD 12:12 or CT1 in DD (value = 1.0). Gene expression was examined in LD 12:12 light conditions for Canton S, cry⁰¹, Pdf ⁰, cry>itp-RNAi, per⁰¹ and in constant darkness (DD) for Canton S. Statistical analyses were carried out to compare differences between time points for each genotype in the same condition. Statistically significant differences are indicated with small letters (A–D) in tables next to the figures. The same letter means no significant differences between those time points.