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. 2015 Sep 1;2015:471468. doi: 10.1155/2015/471468

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Copyright © 2015 Daiki Nagatomo et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Flow cytometry of NALT cells after immunization with TNF/CHP nanoparticles. Mice were nasally administered with IVV (0.3 μg/mouse) or IVV and TNF/CHP nanoparticles (5 μg/mouse as TNF-α) once a week for 3 times. Sixty-eight hours after the last immunization, NALT cells were prepared and subjected to flow cytometric analysis. The expression of CD11c and CD80/CD86 was examined. (a) and (d), saline; (b) and (e), IVV; (c) and (f), IVV with TNF/CHP nanoparticles; (a), (b), and (c), CD80⁺/CD11c⁺; and (d), (e), and (f), CD86⁺/CD11c⁺.