Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 3;6:8200. doi: 10.1038/ncomms9200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(1 and inset) Under basal conditions, DHHC5 is localized to the postsynaptic membrane in complex with PSD-95 and Fyn. PSD-95 binds DHHC5 through PDZ-dependent mechanisms. Fyn binds to the C-terminal poly-proline motif (PTGP) of DHHC5 through its SH3 domain and phosphorylates DHHC5 Y533 within the DHHC5 endocytic motif (YDNL). This inhibits the binding of the endocytic adaptor protein, AP2, to DHHC5. (2) Increased neuronal activity enhances STEP61-mediated dephosphorylation of Fyn and reduces its kinase activity (not shown), as well as decreases DHHC5 association with PSD-95 and Fyn, thereby enhancing DHHC5 internalization and trafficking to dendritic shafts on REs (2–3 min post stimulation). (3) DHHC5 associates with δ-catenin and palmitoylates it (3–10 min post stimulation). (4) DHHC5 and palmitoylated δ-catenin traffic back into spines (3–20 min post stimulation). (5) DHHC5 dissociates from δ-catenin and is reinserted into the synaptic membrane (10–20 min post stimulation). Significantly more DHHC5 is recruited to the synaptic membrane 20 min post stimulation compared with basal levels. δ-Catenin binds and stabilizes N-cadherin, leading to enhancements in synapse structure and increased membrane stabilization of AMPARs 20–60 min post stimulation. The association of δ-catenin with PSD-95 and Fyn, and δ-catenin tyrosine phosphorylation are also increased 20 min post stimulation (not shown), possibly linking cadherin–adhesion complexes with receptor-scaffold assemblies to coordinate synapse strengthening.