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. 2015 Sep 3;6:8200. doi: 10.1038/ncomms9200

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(a) Confocal images of 14–16 DIV neurons transfected with SEP-GluA1, DsRed and the indicated shRNA and HA-DHHC5* constructs (*shRNA-resistance). SEP-fluorescent puncta are pseudocoloured as heat maps. Cells were imaged before and 20 min after glycine stimulation. HA-DHHC5* expression was confirmed by post-hoc immunostaining for HA. Scale bar, 5 μm. (b) IntDen of SEP-GluA1 puncta in hippocampal neurons normalized to the same puncta before glycine treatment. The n-values, indicating neuron numbers from three separate cultures, and the P-values from paired t-tests are as follows: shRNA-c (22, P<0.001), shRNA (18, P=0.769), shRNA+WT* (18, P=0.0009) and shRNA+Y533E* (18, P=0.468). Knockdown of DHHC5 decreased basal SEP-GluA1 IntDen (P=0.0016, F_3,72=5.633, one-way analysis of variance (ANOVA)). Asterisks denote significance between groups before cLTP and relative to shRNA-c, and cross-hatches denote significance within groups before and after cLTP. Graph displays mean±s.e.m. **P<0.01, one-way ANOVA, Tukey's post hoc test; ^###P<0.001, paired t-test.