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. 2015 Sep 15;6:960. doi: 10.3389/fmicb.2015.00960

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Copyright © 2015 Mohiuddin and Schellhorn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic diagram showing water sample fractionation steps and the efficiency of the fractionation scheme. Water samples were separated into three distinct fractions: bacterial, VLP (Virus like particles), and eDNA fraction. eDNA is defined as the DNA released in the environment after lysis (natural decay or lysis) of bacteria, viruses, and higher organisms The efficiency of the fractionation scheme was determined as percent recovery of bacteriophage (MHS 16) mixed with the water sample prior to the fractionation steps.