Figure 7.
Neighbor-joining phylogenetic tree of the capsid assembly protein G20 (T4-like phages or cyanophages) (pfam07230) of Lakeside Beach virome. Lakeside-1 virome library was used as a representative metagenome profile for the Lakeside Beach site. Sequence reads with significant similarity (E-value < 10−3 using blastX) to the G20 marker sequences were obtained, assembled at 98% identity in 35 bp using Cap3 and used to draw the phylogenetic tree alongside reference sequences taken from the protein family (PFAM) database. Sequences for which the best blast hit did not correspond to the G20 marker were excluded from analysis. Bootstrap values of ≥80 are highlighted with black lines.