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. 2015 Sep 15;5:14135. doi: 10.1038/srep14135

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(A,B) Western blot analysis for Shh (27 kDa), Ihh (19 kDa: processed form/42 kDa: precursor form) and Gapdh (36 kDa, as an internal control). Data shown represent one of three experiments with similar results ((A) immunoblot/(B) band intensity). The mean ± SD results obtained by measuring the band density of three different blots are graphed (*p < 0.05, **p < 0.005 vs. control). (C) Immunohistochemistry for Shh and Ihh in liver sections from representative non-Tx and Tx rats at two weeks post transplantation (×40). Shh-positive and -negative hepatocytes are indicated by arrowhead and arrow, respectively. (D) Magnified image of Ihh-positive hepatic oval- and stellate-looking cells indicated by arrowhead and arrow, respectively (×100).