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. 2015 Sep 15;5:14135. doi: 10.1038/srep14135

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Copyright © 2015, Macmillan Publishers Limited

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(A) QRT-PCR analysis of the expression of miRNA-125b, miRNA-324-5p and miRNA-326 in human normal liver (Normal), LX2 (human HSC line) and human CP-MSCs cultured during 72 hours. The mean ± SEM results obtained from three repetitive experiments are graphed (*p < 0.05, **p < 0.005 vs. Normal). (B) In situ hybridization (ISH) with miRNA-125b probe (50 nM) in CP-MSCs and LX2 cultured for 24 hours. ISH with scrambled-miRNA probe (50 nM) in CP-MSCs as a negative control (NC). (C) QRT-PCR analysis of hepatic expressions of miRNA-125b in the livers from all rats. Medians and ranges of results are graphed (*p < 0.05 vs. CON). (D) Representative western blot analysis for CD9 (22 or 24 kDa), an exosomal marker, and GAPDH (36 kDa) in lysates of normal human liver (normal), LX2, and CP-MSCs (Cell lysate) and exosomes from CP-MSC-CM (Exosomes). (E) QRT-PCR analysis for miRNA-125b expression in Normal liver, LX2, CP-MSC lysates, and exosomes produced by CP-MSCs. The mean ± SEM results obtained by three repetitive experiments are graphed (*p < 0.05).