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. 2014 Dec 22;34(37):4808–4820. doi: 10.1038/onc.2014.404

Figure 1.

Figure 1

STIM1 promoted CRC cells invasion and metastasis in vitro. (a1) Expression of STIM1 in four human CRC cell lines relative to the normal human intestinal epithelial cell line (HIEC) was measured by qRT–PCR. (a2) Western blotting analysis of STIM1 expression in different CRC cell lines. (b) After cells were infected with LV-STIM1 (two clones, c1 and c2) or LV-STIM1si-1, -2 or -3, the level of STIM1 protein expression was detected by western blotting analysis. (c) Invasion and migration assay of SW620 cells transfected with LV-STIM1si-1, LV-STIM1si-2 or a negative control. (c1) Representative fields of migration (left) or invasive (right) cells on membrane. (c2) Average cell number of invasion or migration per field from three independent experiments±s.e. *P<0.05. (d1 and d2) Invasion or migration assay of SW480 cells transfected with the LV-STIM1 expression vector (two clones, c1 and c2) or the control vector (NC). Scale bar, 100 μm. *P<0.05.