Figure 1. Characterization of DTS assay.

(A) Schematic process of the DTS assay. Mixture solution including lysis buffer, samples, and DMA is added into the thin film device to extract DNA. The solution is incubated at 56 °C for 20 min to capture DNA through DMA reagent on the surface of the thin film. The DNA is quickly washed and eluted. (B) Water contact angle was measured to check the hydrophilicity change of the surface by APTES modification. (C) Amine-reactive groups on the surface were well-created by APTES at 65 °C for 20 min. (***) indicated statistically significant by the student t-test. All error bars indicate the standard error of the mean based on at least 3 independent experiments. (D) AFM 3D image and the surface roughness confirming the functionality of DNA/DMA complex on the surface.