Figure 3. Application of DTS assay in 12 samples including 6 bacterial cells and 6 eukaryotic cells.

(A) Capacity of the DTS assay with E. coli containing range from 1 × 10³ to 10⁷ colony forming unit (CFU). The colors represent the type of assay; Black (Qiagen kit), and Grey (DTS). The quantity (upper) and purity (lower) of the DNA extracted from E. coli are measured by the spectrophotometer. (B) Capacity of the DTS assay with MCF7 cells containing range from 1 × 10³ to 10⁵ cells. The colors represent the type of assay; Black (Qiagen kit), and Grey (DTS). The quantity (upper) and purity (lower) of the DNA extracted from MCF7 are measured by the spectrophotometer. (C,D) Capacity of the DTS assay with (C) 10⁷ CFU bacterial cells (mycobacteria and salmonella strains) and (D) 10⁵ eukaryotic cells (NCI-H1975: lung, Caco-2: colon, T24: bladder, U937: bone marrow, and Jurkat: lymphocyte). The quantity and purity of the DNA extracted from both eukaryotic and bacterial cells are measured by the spectrophotometer.