Table 1.
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | Amplicon size (bp) |
---|---|---|---|
MiACT | CCCTGAAGAGCACCCA | AGTTGTACGACCACTGGC | 156 |
MiUBI | AAGATCCAGGACAAGGAGG | GGACCAGGTGGAGCG | 125 |
MiTUB | ATCAACTACCAGCCACC | CCTTCCTCCATACCCTCAC | 184 |
MiETR1 | CCAAGGAGAATTGCATGAG | GGCAGCTTGCTCCTC | 141 |
MiERS1 | TGGCGACAAGAAACGACTG | GCCAGTCTCTTGAAGACTC | 116 |
MiERS1m | GCGCTGTAATGAACCATGA | TCTTTGGTATCGTGTTGTC | 151 |
MiERS1s | TCTAGTGTCATGTCTAACTGC | GTGCTACCTTTGTCAAGC | 115 |
β-ACTIN (MiACT), UBIQUITIN (MiUBI), α-TUBULIN (MiTUB), ETHYLENE RESISTANT 1 (MiETR1), ETHYLENE RESPONSE SENSOR 1 (MiERS1), and the two MiERS1 versions MiERS1m and MiERS1s. (Mangifera indica abbreviated as Mi).