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. 2015 Sep 15;6:972. doi: 10.3389/fmicb.2015.00972

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Copyright © 2015 Domröse, Klein, Hage-Hülsmann, Thies, Svensson, Classen, Pietruszka, Jaeger, Drepper and Loeschcke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Strategy for the construction of Pseudomonas putida prodigiosin production strains. (A) The prodigiosin biosynthesis gene cluster from Serratia marcescens is flanked by Tn5 transposon elements, namely a transposase gene as well as transposon outside ends (Tnp-OE), thereby reconstituting a recombinant transposon. (B) The construct is used to create a library of P. putida clones carrying the pig gene cluster at different chromosomal loci. Thus, strongly transcribed chromosomal regions are statistically hit by transposition. (C) P. putida variants, in which strong pig gene transcription results in prodigiosin accumulation, are identified by red color.