Figure 4.

The subcellular localization of 14 FvHsfs. The selected Hsf genes were cloned from a diploid woodland strawberry (F. vesca) and used to construct CaMV35S::Hsfs-GFP vectors in which GFP was fused at the C terminus. The 14 FvHsf-GFP fusion proteins (FvHsfA1b-GFP, FvHsfA1d-GFP, FvHsfA2a-GFP, FvHsfA3a-GFP, FvHsfA4a-GFP, FvHsfA5a-GFP, FvHsfA6a-GFP, FvHsfA9a-GFP, FvHsfB1a-GFP, FvHsfB2a-GFP, FvHsfB2b-GFP, FvHsfB3a-GFP, FvHsfB4a-GFP, and FvHsfC1a-GFP), the VpCDPK2-GFP marker protein, and GFP control were transiently expressed in A. thaliana mesophyll protoplasts and observed by fluorescence microscopy. The merged pictures include the green fluorescence channel (first panels) and the chloroplast autofluorescence channel (second panels). The corresponding bright field images are shown on the right. Bars = 10 μm.