Figure 1. Adoptive transfer of NK1.1⁺ cells isolated from TLR7tg mice.

(A-G) 4x10⁶ sorted NK1.1⁺CD11c⁺ TLR7tg splenoctyes (black bars), or the same number of NK1.1⁺ CD11c⁻ WT splenocytes (white bars) were injected i.v. into a WT recipient. (A) Flow cytometry analysis of NK1.1⁺ sorted cells from WT or TLR7tg. (B) Serum cytokines measured on the first (1w), second (2w) and fourth (4w) week after injection NK1.1 cells; (C) Total serum IgG and anti-RNA antibodies 4 weeks after cell transfer. (D, E) Cells counts of the specified immune populations in the spleen of recipient mice one (D) or four (E) months after injections of NK1.1⁺ cells. Levels of immune populations in WT C57Bl/6 mice are shown as dotted lines in each graph; (F-G) NK1.1⁺ sorted cells from WT (grey bar) or B6.FcγRIIB^−/−Yaa (black bar) spleens were injected i.v. into WT mice and inflammatory cytokines were measured in serum (F) while FACS analysis was performed in the spleen 4 weeks after injection (G). *p<0.05; **p<0.01; ***p<0.001 in Student T-test. Experiments were performed in at least 3 mice per group and repeated 5 times.